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Adiponectin levels are low in obesity, diabetes, and acute alcoholic states. Because the liver is a key regulator of adiponectin expression, we examined the effect of acute alcohol intoxication on liver adiponectin expression. Rats were fed ad libitum, and in a second set of experiments, the nutritional state was altered by 24 h of fasted or refed states. Whole liver RNA was prepared, adiponectin message was quantitated, and adiponectin levels were estimated from 100 microg protein by radioimmunoassay. Acute alcohol intoxicated and fasted and refed-fed rats had significantly lower amounts of hepatic adiponectin than controls. The alcohol-induced decrease was similar to that seen with starvation. Refeeding reversed the decrease to basal values. Hepatic glucocorticoid levels were also low in alcohol-intoxicated and fasted animals. The alcohol-induced decrease in adiponectin was observed with daily administration for 7 days at doses of 0.2-0.4 g/kg body weight. Chronic alcohol treatment did not induce hepatomegaly, but did cause a decrease in the efficiency of adiponectin mRNA translation (2.3-fold). Thus, the acute loss of adiponectin expression seen in alcohol-intoxicated rodents appears to be attributable primarily to reduced levels of adiponectin expression in the liver.HD 49217

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