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Our results clearly demonstrated that overexpression of FAT1 not only decreased the cellular fatty acid content but also accelerated the fatty acid biosynthesis. Therefore, the overexpression of FAT1 accelerates the production of fatty acids in the yeast; thus, the decrease in cellular fatty acid content observed in *Y. lipolytica*overexpressing *FAT1*was expected. It is also obvious that the overexpression of FAT1 affects the substrate preference for the fatty acid biosynthesis in the yeast. Therefore, this study provides a simple assay to screen and identify a heterologous enzyme that has functional activity in the fatty acid biosynthesis of yeasts, such as *S. cerevisiae*, a simple fatty acid auxotroph.

The optimized conditions for cell cultivation employed in this study are appropriate for production of other fatty acids. In particular, unsaturated fatty acids can be synthesized from the common