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The field of the invention is that of analytical techniques used in the detection and analysis of biological substances. This technique is developed in three directions:
(1) analysis of human or animal blood and plasma/serum and of tissues;
(2) analysis of urine and saliva;
(3) detection of chemical agents and
(4) in vitro tests to detect the presence of harmful substances in the body.
Biological substances are a class of heterogeneous macromolecules which include proteins, polysaccharides, steroids and lipids. They are detected by chemical, immunological and biochemical techniques. The distribution and physico-chemical properties of these substances are determined by the type of biological sample used (serum, plasma, tissues or urine and saliva), and also by differences which occur as a function of the organ and/or the type of pathological state. In a toxicological context, for example, the biochemical methods are used in the search for acute or chronic intoxication as a consequence of toxic agents, as well as in the early diagnosis of specific diseases. The levels of the various substances are evaluated by spectrophotometry, densitometry or immunochemistry.
The most common techniques which are used to detect biological substances are immunological and immunochemical methods. They include an enzyme-immunoassay, RIA methods, enzyme linked immunosorbent assays, as well as the known methods of immunochemical and fluorescent measurements.
However, the techniques which are currently used require the presence of an antibody against the substance to be detected. This hinders the development of a large number of tests for substances which have no specific antibodies available in order to detect them.
In the field of detection, it is the aim of the